全文获取类型
收费全文 | 3899篇 |
免费 | 197篇 |
国内免费 | 117篇 |
出版年
2023年 | 37篇 |
2022年 | 43篇 |
2021年 | 118篇 |
2020年 | 105篇 |
2019年 | 72篇 |
2018年 | 75篇 |
2017年 | 87篇 |
2016年 | 92篇 |
2015年 | 124篇 |
2014年 | 146篇 |
2013年 | 296篇 |
2012年 | 157篇 |
2011年 | 151篇 |
2010年 | 149篇 |
2009年 | 143篇 |
2008年 | 165篇 |
2007年 | 164篇 |
2006年 | 141篇 |
2005年 | 144篇 |
2004年 | 140篇 |
2003年 | 126篇 |
2002年 | 124篇 |
2001年 | 102篇 |
2000年 | 102篇 |
1999年 | 104篇 |
1998年 | 103篇 |
1997年 | 101篇 |
1996年 | 75篇 |
1995年 | 87篇 |
1994年 | 86篇 |
1993年 | 95篇 |
1992年 | 77篇 |
1991年 | 63篇 |
1990年 | 37篇 |
1989年 | 48篇 |
1988年 | 36篇 |
1987年 | 38篇 |
1986年 | 29篇 |
1985年 | 28篇 |
1984年 | 31篇 |
1983年 | 31篇 |
1982年 | 32篇 |
1981年 | 25篇 |
1980年 | 23篇 |
1979年 | 17篇 |
1978年 | 14篇 |
1977年 | 10篇 |
1976年 | 15篇 |
1973年 | 2篇 |
1969年 | 1篇 |
排序方式: 共有4213条查询结果,搜索用时 93 毫秒
41.
Summary The pattern of intercellular connections between germ line cells has been studied in follicles of the mutantdicephalic (dic), which possess nurse cell clusters at both poles. Staining of follicles with a fluorescent rhodamine conjugate of phalloidin reveals ring canals and cell membranes and thus allows us to reconstruct the spatial organization of the follicle. Each germ line cell can be identified by the pattern of cell-cell connections which reflect the mitotic history of individual cells in the 16-cell cluster. The results indicate that in both wild-type anddicephalic cystocyte clusters one of the two cells with four ring canals normally becomes the pro-oocyte. However, in some follicles (dicephalic and wild-type) oocytes were found with fewer or more than four ring canals. Indic follicles, one or several nurse cells may become disconnected from the other cells during oocyte growth at stage 9–10. Such disconnected cells cannot later on empty their cytoplasm into the oocyte. This, in turn, might be of consequence for the determination of axial polarity of the embryo. 相似文献
42.
Robert A. Krebs 《Journal of Insect Behavior》1991,4(2):221-233
Variation in copulation duration of Drosophila mojavensisstrains was influenced by both sexes. Males maintained predominant control, as copulation duration of pairs from different strains was more similar to that of the strain from which the male was derived, but female origin also contributed significantly to the duration of copulation. Variation among strains was controlled by genes acting additively in both sexes. The size of both males and females also affected copulation duration. Small males copulated longer on average than large males, while males paired with large females copulated longer than those paired with small females. The importance of copulation duration to fitness was tested by correlation analyses with male size, female size, female remating latency, and number of eggs laid prior to female remating. Longer copulations stimulated earlier oviposition, possibly by increasing accessory gland secretions that are passed by males during copulation. 相似文献
43.
44.
Benjamin Risse Nils Otto Dimitri Berh Xiaoyi Jiang Christian Kl?mbt 《Journal of visualized experiments : JoVE》2014,(94)
The analysis of neuronal network function requires a reliable measurement of behavioral traits. Since the behavior of freely moving animals is variable to a certain degree, many animals have to be analyzed, to obtain statistically significant data. This in turn requires a computer assisted automated quantification of locomotion patterns. To obtain high contrast images of almost translucent and small moving objects, a novel imaging technique based on frustrated total internal reflection called FIM was developed. In this setup, animals are only illuminated with infrared light at the very specific position of contact with the underlying crawling surface. This methodology results in very high contrast images. Subsequently, these high contrast images are processed using established contour tracking algorithms. Based on this, we developed the FIMTrack software, which serves to extract a number of features needed to quantitatively describe a large variety of locomotion characteristics. During the development of this software package, we focused our efforts on an open source architecture allowing the easy addition of further modules. The program operates platform independent and is accompanied by an intuitive GUI guiding the user through data analysis. All locomotion parameter values are given in form of csv files allowing further data analyses. In addition, a Results Viewer integrated into the tracking software provides the opportunity to interactively review and adjust the output, as might be needed during stimulus integration. The power of FIM and FIMTrack is demonstrated by studying the locomotion of Drosophila larvae. 相似文献
45.
46.
47.
Werner Wolfgang Alekos Simoni Carla Gentile Ralf Stanewsky 《Proceedings. Biological sciences / The Royal Society》2013,280(1768)
Circadian clocks are endogenous approximately 24 h oscillators that temporally regulate many physiological and behavioural processes. In order to be beneficial for the organism, these clocks must be synchronized with the environmental cycles on a daily basis. Both light : dark and the concomitant daily temperature cycles (TCs) function as Zeitgeber (‘time giver’) and efficiently entrain circadian clocks. The temperature receptors mediating this synchronization have not been identified. Transient receptor potential (TRP) channels function as thermo-receptors in animals, and here we show that the Pyrexia (Pyx) TRP channel mediates temperature synchronization in Drosophila melanogaster. Pyx is expressed in peripheral sensory organs (chordotonal organs), which previously have been implicated in temperature synchronization. Flies deficient for Pyx function fail to synchronize their behaviour to TCs in the lower range (16–20°C), and this deficit can be partially rescued by introducing a wild-type copy of the pyx gene. Synchronization to higher TCs is not affected, demonstrating a specific role for Pyx at lower temperatures. In addition, pyx mutants speed up their clock after being exposed to TCs. Our results identify the first TRP channel involved in temperature synchronization of circadian clocks. 相似文献
48.
We demonstrate that the lifespan of Drosophila melanogaster population is controllable by a combination of external three-dimensional oscillating low-frequency electric and magnetic fields (3D OLFEMFs). The lifespan was decreased or increased in dependence of the parameters of the external 3D OLFEMFs. We propose that metabolic processes in D. melanogaster’s body are either accelerated (in the case of reduced lifespan) or slowed down (in the case of increased lifespan) in function of 3D OLFEMFs that induce vibrational motions on sub-cellular and larger scales. 相似文献
49.
《Chronobiology international》2013,30(2):77-86
The locomotor activity rhythms of 24 isochromosomal strains of Drosophila melanogaster were recorded in constant conditions, using an experimental design suitable for the serial screening with so many strains using limited equipment. The data obtained were subjected to biometrical genetic analysis. The results show that four characteristics of the rhythms (period, phase, definition, and waveform) display genetically based variation in expression and that each appears to be affected by a range of genes. 相似文献
50.
《Cell cycle (Georgetown, Tex.)》2013,12(17):2753-2759
Over the last decades, it has become clear that glia are multifunctional and plastic cells endowed with key regulatory roles. They control the response to developmental and/or pathological signals, thereby affecting neural proliferation, remodeling, survival, and regeneration. It is, therefore, important to understand the biology of these cells and the molecular mechanisms controlling their development/activity. The fly community has made major breakthroughs by characterizing the bases of gliogenesis and function. Here we describe the regulation and the role of the fly glial determinant. Then, we discuss the impact of the determinant in cell plasticity and differentiation. Finally, we address the conservation of this pathway across evolution. 相似文献